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Author  |
Angeli, J.P.F.; Ribeiro, L.R.; Bellini, M.F.; Mantovani, M.S. |
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Title |
Beta-glucan extracted from the medicinal mushroom Agaricus blazei prevents the genotoxic effects of benzo[a]pyrene in the human hepatoma cell line HepG2 |
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Journal Article |
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Year |
2009 |
Publication |
Archives of Toxicology |
Abbreviated Journal |
Arch Toxicol |
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Volume |
83 |
Issue |
1 |
Pages |
81-86 |
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Abstract |
The mushroom Agaricus blazei is studied for its nutraceutical potential and as a medicinal supplement. The aim of the present study was to investigate the chemoprotective effect of beta-glucan extracted from the mushroom A. blazei against DNA damage induced by benzo[a]pyrene (B[a]P), using the comet assay (genotoxicity) and micronucleus assay with cytokinesis block (mutagenicity) in a human hepatoma cell line (HepG2). To elucidate the possible beta-glucan mechanism of action, desmutagenesis or bioantimutagenesis types, three treatment protocols were tested: simultaneous, pre-treatment, and presimultaneous. The results showed that beta-glucan does not exert genotoxic or mutagenic effect, but that it does protect against DNA damage caused by B[a]P in every protocol tested. The data suggest that beta-glucan acts through binding to B[a]P or the capture of free radicals produced during its activation. On the other hand, the pre-treatment results also suggest the possibility that beta-glucan modulates cell metabolism. |
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Address |
Laboratorio de Genetica Toxicologica, Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, Parana 86951-990, Brazil |
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English |
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ISSN |
1432-0738 |
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Notes |
PMID:18528685 |
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no |
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Call Number |
refbase @ user @ |
Serial |
30 |
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